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. 2014 Oct 28;9(10):e110669. doi: 10.1371/journal.pone.0110669

Figure 6. A. Effect of PKA inhibitor in forward motility of the FMSF activated sperm cells.

Figure 6

Duration of IP20 incubation was 30 min. B. Effect of tyrosine-kinase inhibitor on forward movement profile of FMSF stimulated spermatozoa. Duration of Genistein incubation was 30 min. Viable cell count throughout the different assay conditions remained ≥95%. C. Phospho-kinase stimulating potential of FMSF. Only absorbance values from ELISA results have been plotted, actual activity units have not been shown; anti-phospho-serine/threonine for Protein Kinase A and tyrosine antibodies for Tyrosine kinase were used in ELISA of the assayed treatments. All the data represent mean ± SEM for n = 3 samples. Hash mark (#) indicates statistically significant inhibition vs. only FMSF-treated (p<0.01), Asterisks (*, **) denote statistically significant difference vs. control (p<0.05 and p<0.01), respectively.