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. 2014 Oct 28;9(10):e111423. doi: 10.1371/journal.pone.0111423

Figure 3. The effect of NOX and its ROS products on TACE activity during FSH-induced meiotic resumption in mouse COCs.

Figure 3

COCs were cultured respectively in the following media: (A) FSH-supplemented media with or without TACE selective inhibitor TAPI-2 for 22–24 h; (B) media supplemented with 50 µM TAPI-2, 0.05 IU/mL FSH, TAPI-2 plus FSH, or TAPI-2 plus FSH plus 100 ng/mL Areg for 22–24 h. Figures C and D indicate the TACE activity in cumulus cells of COCs cultured in FSH-supplemented media with or without NOX inhibitor diphenyleneiodonium chloride (DPI, 30 nM), ROS scavenger 1,3-dimethyl-2-thiourea (DMTU, 30 mM) for 0, 2 or 4 h. Letters (a–d) on different columns in each chart indicate a significant difference between groups (p<0.05). AR: Areg.