Figure 4. The effects of (A) GSH, (B) Cys, (C) DTT, and (D) TCEP on the Cys250-Cys32 disulfide bond.
Before analysis, hAS3MT was respectively reduced by GSH (7 mM), Cys (10 mM), DTT (1.5 mM) or TCEP (0.7 mM) for 60 min at 37°C.
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