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(A) in GSH reaction system. (B) in AdoMet control. (C) in hAS3MT. (D) in reduced hAS3MT. (E) MS/MS spectra for the sequence at 1855.13 (m/z) of hAS3MT in GSH reaction system. Labels a, b, c, x, y, and z represent the cleavage manners of peptide. AdoMet control: 1.0 mM AdoMet was incubated with 2.0 µM hAS3MT at 37°C for 60 min.
