Table 1. Sequences for the peptide fragments of hAS3MT analyzed by MALDI-TOF-MS.
Sequence | site | Observed | Mr (expt) | Mr (calc) | ppm |
DCYVLSQLVGEKGHVTGIDMTK | 84–105 | 2450.28 | 2449.27 | 2449.20 | 28.58 |
LGEAGIKNESHDIVVSNCVINLVPDK | 139–164 | 2820.89 | 2819.88 | 2819.45 | 152.49 |
NESHDIVVSNCVINLVPDK | 146–164 | 2152.30 | 2151.29 | 2151.06 | 106.91 |
ELAVLAQKIGFCPPRLVTANLITIQNK | 215–241 | 3008.05 | 3007.04 | 3006.71 | 109.74 |
IGFCPPR | 223–229 | 846.31 | 845.30 | 845.42 | −141.96 |
FAQDFLIRPIGEKLPTSGGCSALELK | 315–340 | 2847.73 | 2846.72 | 2846.50 | 77.28 |
CVPDAAGGCCGTKK | 360–373 | 1366.62 | 1365.61 | 1365.58 | 21.97 |
Values of observed M+H+ (Da) are the results of MALDI-TOF-MS in Figure 1a.