Table 2. Sequences for the peptide fragments of reduced hAS3MT analyzed by MALDI-TOF-MS.
Sequence | site | observed | Mr (expt) | Mr (calc) | ppm |
SADLQTNGCVTTARPVPKHIR | 24–44 | 2320.91 | 2319.90 | 2320.21 | −133.63 |
SADLQTNGCVTTARPVPK | 24–41 | 1914.72 | 1913.71 | 1913.96 | −130.64 |
DCYVLSQLVGEKGHVTGIDMTK | 84–105 | 2449.86 | 2448.85 | 2449.20 | −142.92 |
LGEAGIKNESHDIVVSNCVINLVPDK | 139–164 | 2819.93 | 2818.92 | 2819.45 | −188.02 |
NESHDIVVSNCVINLVPDK | 146–164 | 2151.82 | 2150.81 | 2151.06 | −116.24 |
THKVLWGECLGGALYWKELAVLAQK | 198–222 | 2870.89 | 2869.89 | 2869.53 | 125.44 |
ELAVLAQKIGFCPPRLVTANLITIQNK | 215–241 | 3007.99 | 3006.98 | 3006.71 | 89.79 |
IGFCPPR | 223–229 | 846.27 | 845.26 | 845.42 | −189.29 |
ELERVIGDCRFVSATFR | 242–258 | 2054.82 | 2053.81 | 2054.04 | −111.99 |
ELERVIGDCR | 242–251 | 1246.42 | 1245.41 | 1245.61 | −160.59 |
VIGDCRFVSATFR | 246–258 | 1527.54 | 1526.53 | 1526.77 | −157.22 |
FAQDFLIRPIGEKLPTSGGCSALELK | 315–340 | 2847.06 | 2846.05 | 2846.50 | −158.11 |
CVPDAAGGCCGTKK | 360–373 | 1480.74 | 1479.74 | 1479.63 | 74.34 |
Values of observed M+H+ (Da) are the results of MALDI-TOF-MS in Figure 1b.