Table 3. Sequences for Cys32-Cys250 peptide fragments at 3451.17 (m/z) analyzed by MALDI-TOF-MS/MS.
Sequence | calculated | observed | ppm |
SAD | 247.25 | 247.24 | −40.45 |
IGDCR | 557.63 | 557.61 | −35.87 |
ELERVIGDC | 989.16 | 989.28 | 121.30 |
LERVIGDCR | 1028.23 | 1028.16 | −68.08 |
SADLOTNGCVTTA | 1264.41 | 1264.56 | 118.62 |
VTTARPVPKHIR | 1401.69 | 1401.73 | 28.54 |
ERVIGDCR–GCVTTARPVPKHIR | 2461.96 | 2461.98 | 8.12 |
LERVIGDCR–CVTTARPVPKHIR | 2503.06 | 2503.38 | 127.83 |
DCR–ADLQTNGCVTTARPVPKHIR | 2521.98 | 2522.33 | 138.76 |
ELERVIGDCR–DLQTNGCVTTARPVPKHIR | 3294.82 | 3294.85 | 9.11 |
Values of observed M+H+ (Da) are the results of MALDI-TOF-MS/MS in Figure 1c. Mass for each fragments were calculated according to the standard fragmentation pattern.