Table 5. Sequences for the AdoHcy-Cys250 peptide fragments at 1855.13 (m/z) analyzed by MALDI-TOF-MS/MS.
Sequence | calculated | observed | ppm | Sequence | calculated | observed | ppm |
V | 115.13 | 115.11 | −173.75 | VSATFR | 678.76 | 678.64 | −176.82 |
R | 174.19 | 175.16 | −172.25 | FVSATFR | 810.94 | 810.85 | −111.00 |
VI | 213.29 | 213.25 | −187.57 | RFVSATFR | 1010.13 | 1010.23 | 98.99 |
VIG | 271.34 | 271.39 | 184.24 | CRFVSATFR | 1085.27 | 1085.23 | −36.86 |
FR | 306.37 | 306.32 | −163.23 | DCRFVSATFR | 1200.36 | 1200.54 | 149.93 |
VIGD | 400.43 | 400.36 | −174.84 | GDCRFVSATFR | 1257.41 | 1257.62 | 166.98 |
ATFR | 492.55 | 492.49 | −121.83 | IGDCRFVSATFR | 1371.57 | 1371.80 | 167.66 |
SATFR | 564.63 | 564.56 | −123.99 | DCRFVSATFR-AdoHcy | 1585.36 | 1585.46 | 63.07 |
VIGDCR | 616.77 | 616.74 | −48.64 | IGDCRFVSATFR-AodHcy | 1756.57 | 1755.54 | −17.08 |
Values of observed M+H+ (Da) are the results of MALDI-TOF-MS/MS in Figure 5e. Mass for each fragments were calculated according to the standard fragmentation pattern.