Skip to main content
. 2014 Oct 28;9(10):e110807. doi: 10.1371/journal.pone.0110807

Figure 2. Wild-type Adi3 can be found in endosomal vesicles.

Figure 2

A, Wortmannin and BFA treatment restricts Adi3 to endosomal vesicles. Protoplasts expressing GFP-Adi3 or GFP for 16 hrs were treated with 20 µM FM4-64 for 1 hr, treated with 33 µM wortmannin or 25 µM BFA for 1 hr, and visualized. Compiled Z-axis images are shown. Inlays show zoom of region of interest. Bars = 20 µm. B, Confirmation of GFP-Adi3 protein localization to the endosomal system. The indicated GFP-Adi3 proteins were expressed in protoplasts for 16 hrs and subjected to a sucrose/percoll double gradient and fractionation followed by α-GFP western blot on each fraction. AvrPto-BFP expressed in prf-3 protoplasts for 1 hr was used as plasma membrane marker. Arabidopsis GFP-Ara7 and GFP-Rha1 were used as endosomal markers. Confocal images show representative localization for each construct. AvrPto-BFP false colored red.