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. 2014 Oct 28;9(10):e110807. doi: 10.1371/journal.pone.0110807

Figure 3. AvrPto induces Adi3 endosomal localization.

Figure 3

A, GFP-Adi3 suppression of AvrPto-induced cell death in Nb leaves. GFP-Adi3 or empty vector (yellow dashed circles) were expressed by Agrobacterium-mediated transient expression for 24 hrs followed by expression of AvrPto-FLAG (white dashed circles) so that the two expression zones overlapped. After 72 hrs, leaves were stained with Evans blue, de-pigmented, and photographed. The image presented is representative of 3 independent leaves. Red arrows, zones of Adi3 suppression of AvrPto-induced cell death; black arrows, zones of AvrPto-induced cell death. B, Confocal images of GFP-Adi3 localization in Nb leaf epidermal cells in the presence of vector only (left column) or AvrPto (right column) from regions indicated by red arrows in A. 20 µM FM4-64 was infiltrated and incubated for 2 hrs before imaging. White arrowheads, GFP-Adi3/FM4-64 colocalization. Bars = 20 µm.