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. 2014 Oct 28;9(10):e110545. doi: 10.1371/journal.pone.0110545

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© 2014 Israely et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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BALB/c mice were infected with 4 i.n. ECTV LD₅₀, left untreated or treated on day 3 p.e. and their spleens were photographed (A) and analyzed by flow-cytometry for the distribution and activation (intracellular IFNγ) of various cell populations. (B) Counting of viable lymphocytes was performed under light microscope. (C–H) Number of total and activated cells of the different cell populations: NK (C, D), of CD3⁺ CD4⁺ (E, F) and CD3⁺ CD8⁺ (G, H) cells, respectively.