Figure 1. An APP dimer in 3xK-APP mutant-overexpressing CHO cells.

(A) Schematic of WT-APP and 3xK-APP mutant. (B) Aβ profile analyzed by IP/MS demonstrated increased Aβ42 and Aβ43 levels of the mutant compared to WT. (C) APP dimer bands migrated at ∼200 kDa along with APP monomer bands at ∼100 kDa in a 3–8% tris-acetate gel. Both WT APP and 3xK-APP were normally processed to CTFα and CTFβ, but 3xK-APP produced less CTFα and CTFβ than WT-APP (Also see Figure S1 in File S1).