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| Characteristics | Reference | |
| Strains | ||
| K12 MG1655 | E. coli wild type | [36] |
| Plasmids | ||
| pSB1K3 | BioBrick assembly plasmid | Registry of St. Biological Parts |
| pSB1A1(GFP) | AmpR, λ promoter controlled GFP | This study |
| pKM208 | Red recombinase controlled by lacZ | [16] |
| pCP20 | FLP recombinase helper plasmid | [12] |
| pSB1K3(FRTK) | kanamycin FRT cassette in pSB1K3 | This study |
| pSB1K3(FRTKr) | DNA construct (λ repressor) in pSB1K3(FRTK) | This study |
