Figure 3.

Effect of flurbiprofen on the mRNA expression of TNF-α, IL-6 and IL-1β in the ischemic penumbra of rats. Flurbiprofen was administered at the beginning of reperfusion at the dose of 5 mg/kg and 10 mg/kg. In ischemia/reperfusion and vehicle groups, the mRNA expression of these cytokines was significantly enhanced compared with sham group. Whereas, flurbiprofen can significantly reduce the mRNA expression of these cytokines induced by ischemia/reperfusion insult. The mRNA expression was determined by RT-PCR analysis and bands were quantified by densitometry, normalized to GAPDH levels (A-C). (D) Ischemic penumbra tissue homogenates were prepared to analyze MPO activity by spectrophotometry. Treatment with flurbiprofen at doses of 5 mg/kg and 10 mg/kg significantly relieved the increase of MPO activity induced by ischemia/reperfusion insult. All data were represented as means ± S.D. For each independent experiment, n = 5. #P < 0.05 vs. sham group, *P < 0.05 vs. ischemia/reperfusion and vehicle group, **P < 0.05 vs. FA-L group.