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. 2014 Oct 21;7:1883–1890. doi: 10.2147/OTT.S64339

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© 2014 Qian et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Inhibition of Mus81 by siRNA.

Notes: (A) MCF-7 and T47D cells were treated with FAM-siRNA. Bright-field images are shown in (a), the corresponding fluorescence images are shown in (b), and flow cytometric analysis images are shown in (c). (B) Western blot analysis of Mus81 protein in MCF-7 cells after 24 hours of siRNA transfection. siMus81-3 was the most effective siRNA and was chosen for subsequent study. (C) Western blot analysis of Mus81 protein in MCF-7 cells after 48 and 72 hours of siMus81-3 transfection. (D) Western blot analysis of Mus81 protein in T47D cells after 24, 48, and 72 hours of siMus81-3 transfection. β-Actin was used as loading control.

Abbreviations: siMus81, Mus81 siRNA; siRNA, small interfering RNA; siCtrl, control siRNA; FAM, carboxyl fluorescein.