Skip to main content
. 2014 Oct 28;9(10):e111076. doi: 10.1371/journal.pone.0111076

Figure 7. Nkx2.2 overexpression induces ectopic Olig3 expression.

Figure 7

(A–C) The spinal cords of cE2 chick embryos were electroporated in ovo with replication competent RCASBP retroviral vectors harboring Nkx2.2 gene. The embryos were harvested 2 days later (cE4). Over-expression of Nkx2.2 induced ectopic expression of Olig3 and V3 interneuron marker Sim1. Ectopic Olig3+ and Sim1+ neurons are indicated with arrows. (D) The ratio of the positive cells generated in the Nkx2.2 electroporated (EP) and the control side of the spinal cord. p<0.005. (E–G) Over-expression of Olig3 did not affect Nkx2.2 and Sim1 expression. (H) The ratio of neuron numbers generated on Olig3 electroporated side and the control side. p>0.05. The dorsal part is up. Bars, 100 µm.