OR2 |
82.5 mM NaCl, 2 mM KCl, 1 mM MgCl2, 5 mM HEPES, adjusted to pH 7.4 with NaOH |
ND96 |
96 mM NaCl, 2 mM KCl, 1.8 mM CaCl2, 1 mM MgCl2, 5 mM HEPES, adjusted to pH 7.4 with Tris |
ND9 |
9 mM NaCl, 87 mM NMDG-Cl, 2 mM KCl, 1.8 mM CaCl2, 1 mM MgCl2, 5 mM HEPES, adjusted to pH 7.4 with Tris |
biotinylation buffer |
10 mM triethanolamine, 150 mM NaCl, 2 mM CaCl2, adjusted to pH 9.5 with HCl |
quench buffer |
192 mM glycin, 25 mM Tris-Cl, adjusted to pH 7.5 with HCl |
lysis buffer |
500 mM NaCl, 5 mM EDTA, 50 mM Tris-Cl, adjusted to pH 7.4 with HCl |