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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Br J Haematol. 2014 Sep 8;167(4):487–499. doi: 10.1111/bjh.13066

Figure 2. Efficient delivery of siRNA-αCD22 Ab-SPIO NPs and MXD3 knockdown in preB ALL cells.

Figure 2

(A) Intracellular uptake of the siRNA-αCD22 Ab-SPIO NPs with A488-labelled siRNAs, FITC-labelled αCD22 Abs and A532-labelled SPIO NPs to Reh cells. More nanocomplexes were observed in the cells treated with the nanocomplexes with αCD22 Abs than those without Abs. The images were taken 4 h after treatments.

(B) Addition of αCD22 Abs to the siRNA-αCD22 Ab-SPIO NPs enhancing the efficiency of MXD3 knockdown in Reh cells. Reh cells treated with the nanocomplexes, with or without αCD22 Abs, or left untreated as control, were measured for MXD3 protein expression 4 h after treatments. For each image, the protein level was measured by mean fluorescence intensity (MFI) in each cell in the field and averaged MFI is shown in the right lower corner of each MXD3 image.

αCD22 Ab, anti-CD22 antibody; SPIO, superparamagnetic iron oxide; NP, nanoparticle; siRNA, small interfering RNA.