Combination single treatment of the MXD3 siRNA-αCD22 Ab-SPIO NPs and doxorubicin or vincristine at the 50% inhibitory concentration (IC50) doses showed significantly enhanced cytotoxicity in Reh cells. Data were collected in triplicates, with 2 independent experiments (n = 6). Data as mean. MXD3 siRNA-αCD22 Ab-SPIO NPs only vs. MXD3 siRNA-αCD22 Ab-SPIO NPs + doxorubicin (****) p < 0.0000001, doxorubicin only vs. MXD3 siRNA-αCD22 Ab-SPIO NPs + doxorubicin (****) p = 0.0000032, MXD3 siRNA-αCD22 Ab-SPIO NPs only vs. MXD3 siRNA-αCD22 Ab-SPIO NPs + vincristine (****) p < 0.0001 and vincristine only vs. MXD3 siRNA-αCD22 Ab-SPIO NPs + vincristine (****) p < 0.0000001.
SPIO, superparamagnetic iron oxide; NP, nanoparticle; siRNA, small interfering RNA.