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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Br J Haematol. 2014 Sep 8;167(4):487–499. doi: 10.1111/bjh.13066

Table I.

Efficiency of siRNA and αCD22 antibody loading onto SPIO nanoparticles

APC
Absorbance % Intensity
αCD22 Ab-SPIO pellet 1.20E+06 89.9
supernatant (unbound αCD22 Ab) 1.35E+05 10.1
siRNA-αCD22 Ab-SPIO pellet 6.89E+05 47.1
supernatant (unbound αCD22 Ab) 7.74E+05 52.9

Alexa488
Absorbance % Intensity

siRNA-SPIO pellet 6.06E+07 95.3
supernatant (unbound siRNA) 2.96E+06 4.66
siRNA-αCD22 Ab-SPIO pellet 5.58E+07 100
supernatant (unbound siRNA) 1.27E+03 0

APC, allophycocyanin; Alexa488, Alexa Fluor 488; αCD22 Ab, anti-CD22 antibody; SPIO, superparamagnetic iron oxide; siRNA, small interfering RNA.