Figure 8.

Effects of CXCR4/ACKR3 ligands on vascular α₁-AR reactivity do not depend on the endothelium. (A) Histology (H&E staining) of normal mesenteric arteries (left) and arteries after mechanical endothelial denudation (right). Arrows indicate endothelium. Scale bars: 100 μm. (B–D) Mesenteric arteries with (+) and without (−) endothelium were first constricted with the EC₅₀ dose of phenylephrine (1 μmol/L), followed by addition of the endothelium-dependent vasodilator carbachol (10 μmol/L). Following washout of phenylephrine and carbachol, arteries were reexposed to 1 μmol/L phenylephrine and the effects of (B) TC14012, (C) CXCL12 and (D) ubiquitin (Ub) tested; 10 μmol/L each, n = 3. The effects of the CXCR4/ACKR3 modulators are expressed as % change of i.d.