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Nrf2–HO-1 axis regulates macrophage differentiation and function in vitro. Nrf2-deficient BMMs were transfected with Ad-HO-1 or Ad-β-gal (at an MOI of 10). After 36–48 h, cells were incubated with LPS (100 ng/mL) for an additional 6 h. Quantitative RT-PCR assisted analysis of macrophage: (A) phenotype (Nos2+, Mrc-1/Arg-1+); (B) TGF-β/IL-10 levels. *p < 0.05. Mean ± SD; n = 3–4/group.
