Figure 2.

The concentration of NTBI measured in haem solutions of 10 μmol/L (dark grey columns), 20 μmol/L (light grey columns) and 50 μmol/L (medium grey columns) following incubation for 30 minutes at room temperature without H₂O₂ (unoxidised), 1 mM H₂O₂ and 5 mM H₂O₂.

Results are expressed as the mean±SD of five experiments. There was very little NTBI in the non-oxidised group, and no significant differences with regard to haem concentration in this group. In the oxidised groups the level of NTBI increased with haem concentration (p<0.05). ANOVA with Duncan’s multiple comparison test.