Table I.
The effect of time of incubation with hydrogen peroxide on the release of NTBI from haem.
| Time (min) | NTBI μmol/L | |
|---|---|---|
|
| ||
| 1 mM H2O2 | 5 mM H2O2 | |
| 30 | 1.301±0.166 | 8.326±0.120 |
| 60 | 1.787±0.084 | 9.347±0.467 |
| 90 | 1.748±0.119 | 11.049±0.159 |
| 120 | 1.673±0.056 | 12.051±0.297 |
The amount of NTBI released from 50 μM haem solutions as a function of incubation time. Solutions were incubated with either 1 mM H2O2 or 5 mM H2O2 for 30–120 minutes at room temperature. Results are expressed as the mean±SD of five separate experiments. There were no significant differences in the 1 mM group with the exception of times 30 and 60 minutes. The NTBI levels increased significantly (p<0.05) with incubation time in the 5 mM group. ANOVA with Duncan’s multiple comparison test.