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Effect of CSR on TJ protein expression. A, Several TJ proteins showed decreased mRNA expression in the CSR group, including occludin, claudin-1, claudin-5, and ZO-2; **p < 0.01, ***p < 0.005. B, The immunofluorescence of occludin in freshly isolated cerebral microvessels showed line-like paracellular distribution in the naive group, was decreased in the CSR group, and returned to the control level after 24 h of recovery sleep. Scale bar, 5 μm.
