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. 2014 Oct 25;12:59. doi: 10.1186/s12964-014-0059-5

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© Shang et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of CLDN4 on EphA2 expression. (A) Representative Western blot showing that CLDN4 knockdown increased EphA2 level, and that this was partially reversed by re-expression of CLDN4 expression. (B) The histogram shows the mean level of EphA2 protein determined from 3 independent experiments expressed as the fold change relative to that in the scrambled siRNA control 2008/SCB cells after normalization to β-actin. (C) qRT-PCR analysis showing that knockdown of CLDN4 increased EphA2 mRNA level and that this was partially reversed by re-expression of CLDN4. Results are given as mean ± SEM (n = 3–4). ^** p < 0.01 versus 2008/SCB; ^## p < 0.01 versus CLDN4KD.