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. 2014 Oct 25;12:59. doi: 10.1186/s12964-014-0059-5

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© Shang et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of CLDN4 knockdown on EphA intracellular distribution. (A) The distribution of EphA2 in the 2008/SCB, CLDN4KD and CLDN4KD/rescued cells was visualized by immunofluorescent staining using anti-EphA2 antibody (green). Cell nuclei were counterstained with DAPI (blue). (B) Detection of EphA2 in biotinylated plasma membrane preparations by Western blot analysis using anti-EphA2 and anti-transferrin receptor antibody to provide a lane loading control. (C) Histogram summarizing the results of four independent Western blots.