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. 2014 Oct 25;12:59. doi: 10.1186/s12964-014-0059-5

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© Shang et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Re-expression of E-cadherin in CLDN4KD cells does not significantly reduce total or pEphA2-Ser897. (A) Western blot documenting increased expression of total E-cadherin in CLDN4KD/Ecad cells. (B) Representative Western blot showing total EphA2 levels in CLDN4KD/EV and CLDN4KD/Ecad cells. (C) The histogram shows the mean level of total EphA2 protein determined from 3 independent experiments expressed as the fold change relative to that in the empty-vector transfected control CLDN4KD/EV cells after normalization to β-actin. (D) Representative Western blot showing phosph-EphA2 levels in CLDN4KD/EV and CLDN4KD/Ecad cells. (E) The histogram shows the mean phospho-EphA2/total EphA2 ratio in CLDN4KD/EV and CLDN4KD/Ecad cells determined from 3 independent experiments. The data are expressed as mean ± SEM (n = 3).