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. 2014 Oct 25;12:59. doi: 10.1186/s12964-014-0059-5

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© Shang et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Over-expression of CLDN4 diminishes EphA2 oncogenic signaling even in the absence of E-cadherin. (A) Western blot analysis confirming increased expression of CLDN4 in HEY/mc-CLDN4 cells. (B) qRT-PCR analysis of the effect of CLDN4 over-expression on EphA2 mRNA levels. (C) Representative Western blot showing the effect of over-expression of CLDN4 in HEY cells on total EphA2 protein levels and EphA2 phosphorylation. (D) The histogram shows the mean level of the protein determined from 3 independent experiments. The relative total EphA2 levels in the HEY/mc-CLDN4 cells was expressed as the fold change relative to that in the HEY/mc cells after normalization to β-actin. The extent of EphA2 phosphorylation in the HEY/mc-CLDN4 cells was expressed as the ratio relative to that in the control HEY/mc cells after normalization to respective total EphA2 levels. Results presented are mean ± SEM (n = 3). ^* p < 0.05 versus HEY/mc.