Figure 1. Development of anchorage-independent multicellular spheroids and induction of reversal into monolayer.

Upper panel: A schematic showing current in vitro models and the proposed in vitro model. Lower panel: (a) The parental cell line, Huh7 as monolayer. (b,c) Anchorage-independent growth of multicellular spheroids shown at low (b) and high magnifications (c). (d–f) Induction of reversal of a spheroid into monolayer at increasing magnifications. (g) Progressive reversal of a spheroid over few days to reach complete reversal.