Figure 3. Dynamics of glucose-d₇ derived lipogenesis in PANC1 cells.

(a). Monitoring de-novo lipogenesis in PANC1 cells over time by SRS imaging at C–D and C–H vibration. Inset of image at 72 h: zoom in image of marked area showing the donut-shaped newly synthesized LDs. (b). Quantification of SRS signal at C–D vibration per cell. Data were shown as Mean ± SD. N = 5 for each group. (c). SRS imaging of PANC1 cells incubated with 10 mM 2-DG and 25 mM glucose-d₇ for 3 days. (d). SRS imaging of PANC1 cells incubated with glucose-d₇ for 3 days and treated with DMSO or 20 μM FAS inhibitor C75 for 24 hours. (e). SRS imaging of PANC1 cells stably transfected with FAS shRNA and incubated with glucose-d₇ for 3 days.