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. 2014 Oct 23;16(10):824–834. doi: 10.1016/j.neo.2014.08.006

Figure 1.

Figure 1

Effect of A77 1726 on the PI3K and MAP kinase pathways. A375 cells seeded in a six-well plate were starved in DMEM containing 0.5% FBS for 2 hours and treated with the indicated concentration of A77 1726 for another 2 hours (A) or treated with A77 1726 (100 μM) for the indicated time (B). Cells were harvested and analyzed for protein phosphorylation by specific antibodies as indicated. Protein loading was monitored by stripping membrane and reprobing with antibodies against non-phosphorylated proteins.