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. 2014 Oct 23;16(10):824–834. doi: 10.1016/j.neo.2014.08.006

Figure 5.

Figure 5

Effect of exogenous uridine and MAP kinase pathway inhibitors on A77 1726–stimulated S phase entry and cell cycle arrest. (A) Ability of uridine to relieve the cell cycle arrest in the S phase. A375 cells grown in six-well plates were treated with the indicated concentration of A771726 with or without uridine (200 μM) for 24 hours. Cell cycle was analyzed in a flow cytometer as described in the Materials and Methods section. (B and C) Effect of the MAP kinase pathway inhibitors (B) and the PI3K pathway inhibitors (C) on A77 1726–mediated cell cycle arrest in the S phase. A375 cells were treated with A771726 (100 μM) in the absence or presence of 0.1% DMSO, PLX 4720 (1 μM), U0126 (10 μM) (B), or rapamycin (20 nM) and LY294002 (10 μM) (C) for 24 hours. Single-cell suspensions were prepared and analyzed for cell cycle in a flow cytometer.