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. 2014 Oct 23;16(10):835–844. doi: 10.1016/j.neo.2014.08.008

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© 2014 Neoplasia Press, Inc. Published by Elsevier Inc.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

PMC Copyright notice

Effect of Dbait on cell survival and repair of DNA breaks induced by RT. (A) Cell survival assay. Transfected SK28 and 501mel cells were irradiated with 1 to 8 Gy, and clonogenic survival was estimated 12 days later. (B) Simulation of expected additive effect of Dbait and irradiation on cell survival. Data are represented as median values ± standard error. (C) The extent of DNA damage in transfected cells was analyzed by alkaline Comet assay at various times (0, 60, and 180 minutes and 24 hours) after Dbait or control treatment and at various times (0, 5, 30, 60, and 180 minutes and 24 hours) after combination with irradiation (8 Gy) in SK28 and 501mel cell lines.