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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Eur Respir J. 2014 Mar 6;44(1):109–121. doi: 10.1183/09031936.00075713

FIGURE 6.

FIGURE 6

Impact of in vivo neutralization of TGF-β signalling on hyperoxia-induced transglutaminase gene expression. Gene expression of mRNA encoding a) tgm1 and b) tgm2 was assessed in the lungs of mouse pups at post natal day 10.5, which inspired 21% O2 (open bars) or 85% O2 (grey bars) from P0.5, and were treated with a pan TGF-β1,2,3-neutralizing IgG (1D11) or with an isotype-matched control (MOPC21) IgG. The mRNA levels were assessed by real-time RT-PCR in mRNA pools from whole-lung homogenates, using the primers in table 1, with the 18s rRNA serving as a reference. The numbers above the brackets indicate the p values, which were assessed by unpaired Student’s t-test, and were used to compare the 21% O2 and 85% O2 groups, or to compare the MOPC21-treated and 1D11-treated normoxia groups (n = 6; per group).