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. Author manuscript; available in PMC: 2014 Oct 29.
Published in final edited form as: Nat Commun. 2011 Oct 11;2:496. doi: 10.1038/ncomms1502

Figure 6. Lateral line placodes give rise to ampullary organs and neuromasts.

Figure 6

(a) Stage 32 embryo after focal injection with DiI in the anterodorsal placode; injection site outlined in red. (b) Same embryo at stage 46, showing DiI-labelled cells in the infraorbital neuromasts and dorsal infraorbital and ventral infraorbital ampullary organ fields. (c, d) Transverse sections through (c) a DiI (red)-labelled neuromast and (d) DiI-labelled ampullary organs, counterstained with Sytox Green. (e) Stage 32 embryo after focal injection with DiI in the anteroventral placode; injection site outlined in red. (f) Same embryo at stage 46, showing DiI-labelled cells in preopercular neuromasts and flanking ampullary organs. (g) Opercular skin mount from embryo in (f), counterstained with Sytox Green, showing DiI in preopercular neuromasts and flanking ampullary organs. (h) Magnification of DiI-labelled neuromasts from preopercular neuromast line in (g). (i) Magnification of DiI-labelled ampullary organ from posterior preopercular field in (g). The plane of focus is the sensory cell epithelium. (j) Stage 34 embryo after focal injection with DiI in the anteroventral placode; injection site outlined in red. (k) Same embryo at stage 46, showing DiI-labelled cells in a subset of preopercular neuromasts and flanking ampullary organs. (l) Confocal image of transverse section from embryo in (k), stained with phalloidin (green) and DAPI (blue), showing a DiI (red)-labelled ampullary organ. (m) Magnification from (l), showing DiI (red) within the sensory epithelium. (n) Stage 32 embryo after focal injection with DiI in the anterodorsal placode; injection site outlined in red. (o) Same embryo at stage 46, showing DiI-labelled cells in infraorbital line neuromasts and flanking ampullary organs. (p) Transverse section through head of embryo in (o), stained with Sytox Green. (q) Magnification from (p) showing DiI-labelled cells (red) in the anterodorsal lateral line ganglion. Scale bars: 500 μm (a, e, j, n), 1 mm (b, f, k, o), 50 μm (c, d, h, i, q), 100 μm (g), 30 μm (l), 250 μm (p). Abbreviations: ao, ampullary organ; e, eye; GAD, anterodorsal lateral line ganglion; hb, hindbrain; io, infraorbital lateral line; nm, neuromast; ov, otic vesicle; p, pharynx; pop, preopercular lateral line.