Table 1.
Homeobox Gene Expression Profile in First-Trimester Chorionic Villous Stroma and in Isolated Term CMSCs.
| First Trimester Chorionic Villous Stroma. Average Signal Intensitya | Gene Symbolb | Gene Namec | Term-CMSCs. Relative Quantitation by Real-Time PCRd |
|---|---|---|---|
| 3800.35 | ZHX1 | Zinc finger and homeoboxes 1 | 1.85 |
| 2248.10 | MEIS2* | Meis1, myeloid ectropic viral integration site 1 homolog 2 | 1.05 |
| 728.60 | HLX* | H2.0-like Drosophila | 0.44 |
| 482.20 | MSX2* | Msh homeobox 2 | 0.52 |
| 415.10 | LIM6 | LIM-domain binding 6 | 0.70 |
| 400.53 | MOX2 | Mesenchyme homeobox 2 | 0.61 |
| 387.83 | HHEX* | Hematopoietically expressed homeobox | 1.29 |
| 363.83 | TGIF* | TGFB-induced factor | 1.24 |
| 339.90 | HOXA9 | Homeobox A9 | 1.1 |
| 258.80 | PHOX2A | Paired-like (aristaless) homeobox 2a | 0.70 |
| 254.27 | HOXB7* | Homeobox B7 | 0.56 |
| 229.40 | VSX1 | Visual system homeobox 1 homolog, CHX10-like | 0.80 |
| 223.33 | NKX3-1 | NK3 transcription factor related, locus 1 | 0.52 |
| 157.70 | DLX5* | Distal-less homeobox 5 | 0.57 |
| 154.80 | DLX4* | Distal-less homeobox 4 | 0.61 |
| 128.70 | PRRX1 | Pared related homeobox 1 | 0.39 |
| 124.00 | SIX6 | Sine oculis homeobox homolog 6 | 0.41 |
| 104.20 | PITX1 | Paried-like homeodomain transcription factor 1 | 0.66 |
Abbreviations: CMSCs, chorionic mesenchymal stem/stromal cells; PCR, polymerase chain reaction; TGFB, transforming growth factor β; mRNA, messenger RNA; rRNA, ribosomal RNA.
a Average signal intensity above the arbitrary cutoff of 100.
b Gene symbol with asterisk represents a gene chosen for further mRNA/protein analysis.
c Gene name.
d Real-time PCR was used to detect homeobox gene mRNA relative to 18S rRNA in term CMSC.