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. 2014 Sep;21(9):1187–1195. doi: 10.1177/1933719114542010

Figure 1.

Figure 1.

Celecoxib increased cell proliferation and cyclooxygenase 2 (COX-2) expression. Leiomyoma cells obtained from 5 patients were cultured and treated with prostaglandin E2 (PGE2; 10 μmol/L). A, At 72 hours, cells were subjected to cell proliferation assays by (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). PGE2 (10 μmol/L) induced cell proliferation, which was not inhibited by treatment with 1 μmol/L progesterone (P4). However, treatment with 10 μmol/L COX-2 inhibitor, celecoxib, decreased cell proliferation. B, Western blotting after 6 hours treatment. COX-2 expression was decreased by celecoxib treatment, compared with PGE2 and/or P4 treatment. C, Expression of COX-2 messenger RNA in leiomyoma cells. The results are shown as arbitrary densitometric units (COX-2/GAPDH). The MTT assay was performed at 72 hours to compare the viability among the 4 treated groups of cell. The bars represent the mean ± standard error of values from triplicate experiments. a* indicates significant change relative to control and b* indicates significant change relative to PGE2-treated cells. *P < .05.