Figure 3.

Leiomyoma cells (n = 5) were treated with prostaglandin E2 (PGE2) for 0, 0.5, 1, 2, and 4 hours, and the levels of nuclear factor κB (NF-κB) were measured by Western blots. A, Western blot after 2 hours treatment. PGE2 induced translocation of NF-κB to nucleus and degradation of I-κB in cytosol, which was inhibited by celecoxib but not P4. B, Graphical representation of nucleic NF-κB and cytosolic I-κB expressions by densitometry. The data are expressed as arbitrary units. The bars represent the mean ± standard error of values from triplicate experiments. a* indicates significant change relative to control and b* indicates significant change relative to PGE2-treated cells. *P < .05.