Figure 4.

The messenger RNA (mRNA) levels of proinflammatory cytokines, growth factors, and extracellular matrix were measured by quantitative polymerase chain reaction from leiomyoma cells (n = 5). A, Interleukin 6 and tumor necrosis factor α mRNA expressions were decreased in the cells treated with P + C for 2 hours. B, On the third day of treatment, expressions of platelet-derived growth factor, epidermal growth factor, and transforming growth factor β were decreased by celecoxib, compared to prostaglandin E2 (PGE2) or progesterone. C, Increased expressions of collagen A and fibronectin induced by PGE2 and/or P4 were reduced by celecoxib treatment. The data are expressed as arbitrary units. The bars represent the mean ± standard error of values from triplicate experiments. N indicates non-treated; P + P4, prostaglandin E2 plus progesterone; P + C, prostaglandin E2 plus celecoxib. a* indicates significant change relative to control and b* indicates significant change relative to PGE2-treated cells. *P < .05.