Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 13;1(2):88–98. doi: 10.1002/acn3.28

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 The Authors. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc on behalf of American Neurological Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Heterozygous deletion of CERS2 reduces CERS2 expression, CERS2 protein level, and activity in fibroblasts. (A) Total RNA was isolated from cultured fibroblasts from the proband and controls and expression levels of CERS1-6 were determined by quantitative real-time PCR. Mean ± SD is shown, N = 3. Statistical analyses were performed using a multiple t-test with significance levels: *(P < 0.05), **(P < 0.01), and ***(P < 0.001). (B) Total cell extracts were prepared from cultured fibroblasts and CERS2 levels were determined by Western blotting. TFIIB served as loading control. (C) Ceramide synthase activities were determined in whole cell extracts using the indicated acyl-CoAs as substrates. Mean ± SEM is shown, N = 6–9. Statistical analyses were performed using multiple t-test with significance levels: *(P < 0.05) and **(P < 0.01).