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. 2014 Jan 13;1(2):88–98. doi: 10.1002/acn3.28

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© 2014 The Authors. Annals of Clinical and Translational Neurology published by Wiley Periodicals, Inc on behalf of American Neurological Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Lipid profile of glycosylceramide species in proband fibroblasts. Lipid extracts, prepared from cultured fibroblasts were analyzed using LC-MS. Glycosylceramide species containing long-chain fatty acids are shown in (A), while glycosylceramide species with very long-chain fatty acids are shown in (B). Lipids levels were normalized to total cell number and internal standards. Three independent cultures of fibroblasts from the proband and controls were analyzed. Mean ± SD is shown, N = 3. Statistical analyses were performed using multiple t-test with significance levels: *(P < 0.05) and **(P < 0.01).