Figure 6.

Cholera toxin B labeling of fibroblasts from proband and parental controls. (A) Fibroblasts from proband and controls were stained with Alexa488-labeled cholera toxin B for 20 min., and subsequently examined by fluorescence microscopy. (B) Images and fluorescence intensities were analyzed using ImageJ. Mean ± SEM is shown, N (parent 2) = 38 and N (proband) = 49. Statistical analyses were performed using unpaired two-tailed t-test with Welch's correction with significance level: ***(P = 0.0004).