Table 1.

Primers for Amplification of HPV Genome.

Primer	Sequence	Location on Virus Genome	Length (bp)
HPV16 E6 S (20 mer)	5′-GCA ATG TTT CAG GAC CCA CA-3′ (forward)	101-219	119
HPV16 E6 AS (22 mer)	5′-CGC AGT AAC TGT TGC TTG CAG T-3′ (reverse)
HPV16 E7 S (23 mer)	5′-TTG TTG CAA GTG TGA CTC TAC GC-3′ (forward)	732-824	93
HPV16 E7 AS (23 mer)	5′- CCT AGT GTG CCC ATT AAC AGG TC-3′ (reverse)
HPV18 E6 S (21 mer)	5′-TCA CAA CAT AGC TGG GCA CTA-3′ (forward)	485-576	92
HPV18 E6 AS (21 mer)	5′-CTT GTG TTT CTC TGC GTC GTT-3′ (reverse)
HPV18 E7 S (21 mer)	5′-ATG AAA TTC CGG TTG ACC TTC-3′ (forward)	645-746	102
HPV18 E7 AS (21 mer)	5′-GTC GGG CTG GTA AAT GTT GAT-3′ (reverse)
β-actin 1-S (20 mer)	5′-GGC ATC CTC ACC CTG AAG TA-3′ (forward)	3198-3279	82
β-actin 1-AS (20 mer)	5′-AGG TGT GGT GCC AGA TTT TC-3′ (reverse)

bp = base pairs.