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. Author manuscript; available in PMC: 2014 Oct 29.
Published in final edited form as: Eur J Immunol. 2009 Jul;39(7):1726–1735. doi: 10.1002/eji.200839001

Figure 6.

Figure 6

The NKT-cell hybridoma XV19 was autoreactive toward splenocytes lacking sulfatide. Indicated numbers of splenocytes from CST−/− (black squares) and CST+/− littermate control mice (white triangles) were cultured together with the XV19 NKT-cell hybridoma for 16 h, after which IL-2 secretion was assayed using CTLL-2 cells. The cultures were performed in the absence of serum (A) or in regular supplemented RPMI with 5% FBS (B). Data shown are one representative experiment of three (B) or one (A) performed (mean ± SD of triplicate cultures). (C) XV19 cells were tested for their reactivity to a number of different GSL (see Table 2) by pulsing JawsII cells (5 × 103 cells/well) with the indicated GSL concentrations, before the addition of hybridoma cells. IL-2 secretion was assayed using CTLL-2 cells. Data are representative of two experiments using JawsII and two using RMA-S as APC.