Pulp Revascularization of Immature Permanent Teeth: A Review of the Literature and a Proposal of a New Clinical Protocol

. 2014 Oct 14;2014:737503. doi: 10.1155/2014/737503

(b)

Second step
(two or three weeks later if asymptomatic tooth and/or absence of fistula)
Local anesthesia without vasoconstrictor^a
Isolation of the tooth with a rubber dam
Disinfection of the tooth with 10% povidone-iodine (iso-Betadine) before opening it^b
Opening the tooth to have a access to root canal
Removal of the triple antibiotic paste using irrigation with sodium hypochlorite (1.25%–5.25%) then with physiological serum and finally with 2% chlorhexidine^c
An apical bleeding is caused. Blood level must be at the cement-enamel junction.
Preparation of mineral trioxide aggregate (MTA) and its placement on the clot^d in order to form a hermetic sealing
Place a wet a cotton ball on MTA filling
Sealing of the cavity with a temporary filling

^aIn order to not inhibit the future apical bleeding.

^bAccording to the authors, disinfection is done or not.

^cIrrigation is done in order to make space for the future blood clot.

^dIt takes 15 minutes to obtain a blood clot.