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. Author manuscript; available in PMC: 2015 Mar 18.
Published in final edited form as: Nat Commun. 2014 Sep 18;5:4954. doi: 10.1038/ncomms5954

Figure 5. TBR1 homodimerizes.

Figure 5

(a) BRET assays for interaction between WT and mutant TBR1 proteins. (b) Schematic representation of synthetic TBR1 variants. (c) Immunoblot of YFP–TBR1 fusion proteins in transfected HEK293 cells. (d) Fluorescence microscopy images of HEK293 cells transfected with synthetic TBR1 variants fused to YFP (shown in green). Nuclei were stained with Hoechst 33342 (blue). Arrows indicate protein in the cytoplasm. Scale bar, 20 μm. Concordant results were seen in SHSY5Y cells, as shown in Supplementary Fig. 2. (e) BRET assays for interaction between WT TBR1 and synthetic truncations. For a and e bars represent the mean corrected BRET ratios±s.e.m. of one experiment performed in triplicate. BRET assays were performed in HEK293 cells.