Table 3.
Nutrient effects on transformation frequencies of V. eiseniae 05-2r.
| Supplemented nutrienta | Trial 1b | Trial 2b |
|---|---|---|
| MSM | 8.6 × 10-4 ± 2.0 × 10-4 (X) | 6.6 × 10-4 ± 2.2 × 10-5 (A) |
| ACM | 4.2 × 10-4 ± 1.2 × 10-4 (Y) | |
| NH4Cl | 9.3 × 10-4 ± 1.1 × 10-4 (X) | |
| D-mannose | 4.8 × 10-4 ± 1.1 × 10-4 (Y) | 3.2 × 10-4 ± 5.6 × 10-5 (B) |
| Galactose | 6.1 × 10-4 ± 7.7 × 10-5 (A) | |
| Fructose | 2.2 × 10-4 ± 1.4 × 10-4 (BC) | |
| L-fucose | 2.2 × 10-4 ± 2.6 × 10-5 (BC) | |
| D-arabinose | 1.4 × 10-4 ± 1.1 × 10-4 (CD) | |
| β-Hydroxybutyrate | 9.1 × 10-5 ± 9.0 × 10-5 (D) | |
| Pyruvate | 2.7 × 10-5 ± 9.0 × 10-6 (D) |
a
Nutrients added to a concentration of 20 mM. 20 ng pENTR/D-Topo-MCS:kanpilBC added to 30 μl reaction incubated at 25∘C for 24 h. Cell density adjusted to OD600 of 1.
b
Mean transformation frequency ± SD, n = 4. Values with different letters differ significantly (p < 0.05).