Table 4.
Natural transformation of TFP mutants.
| Strain | TFPa | Transformation frequencyb |
|---|---|---|
| EF05-2r | + | 1.2 × 10-2 ± 5.2 × 10-3 (A) |
| VEpilBC- | - | 1.3 × 10-7 ± 7.3 × 10-8 (C) |
| VEpilT- | ++ | 3.2 × 10-8 ± 3.2 × 10-8 (C) |
| VEflgKL- | + | 5.2 × 10-3 ± 1.1 × 10-3 (B) |
a
+, Presence of TFP; ++, hyper-piliated; -, no TFP; presence of TFP confirmed by TEM.
b
Mean transformation frequency ± SD, n = 4. Strains tested with 20 ng pENTR/D-Topo-MCS:gentptsI/ 30 μl MSM. Values with different letters differ significantly (p < 0.05).