Figure 3.

TOLLIP is found in primary human nasal, bronchial and alveolar epithelial cells. Primary nasal (A and B), bronchial (C and D) and type II alveolar epithelial cells (E and F) were fixed, blocked with 2% goat serum and incubated with a rabbit polyclonal antibody against TOLLIP (A, C and E) or isotype control (B, D and F). Nuclei were stained with DAPI (blue). Secondary antibody was antirabbit IgG conjugated with Alexa 488 (green). Images were analysed using confocal microscopy. Three nasal samples, one bronchial and one alveolar were analysed. Scale bar equals 50 μm in A–D, and 10 μm in E and F (TOLLIP, Toll-interacting protein).